A common barrier to high-resolution structure determination is the growth of large well-ordered crystals. Electron diffraction is capable of producing high-quality diffraction data from crystals that are orders of magnitude smaller than those needed for conventional X-ray crystallographic experiments, and 3D electron diffraction methods have recently begun to yield high-resolution structures from extremely small microcrystals. In this presentation, the cryo-electron microscopy technique of microelectron diffraction, or MicroED, will be described in detail along with representative structures determined by the method. Additionally, current work in our lab, which is focused on improving and extending this technique to very difficult samples, such as membrane proteins, will be presented.
Arizona State University
Wednesday, February 27, 2019